Human Growth Associated Protein 43 (GAP-43) ELISA Kit

Cat.#:

JL11115

Regulatory Status:

RUO

Alternative:

F1; B-50; PP46; Neuron Growth-Associated Protein 43; Neuromodulin; Axonal Membrane Protein GAP-43; Protein F1; Calmodulin-Binding Protein P-57; Neural Phosphoprotein B-50

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.075ng/mL

Specificity:

This kit recognizes Human GAP-43 in samples. No significant cross-reactivity or interference between Human GAP-43 and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Developmental Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| GAP-43

Growth Associated Protein 43 (GAP43) is a protein encoded by the GAP43 gene. GAP43 is called a "growth" or "plasticity" protein because it is expressed at high levels in neuronal growth cones during development[6] and axonal regeneration, and it is phosphorylated after long-term potentiation and after learning. GAP43 is a crucial component of the axon and presynaptic terminal. Its null mutation leads to death within days after birth, due to axon pathfinding defects.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.29	1.29	0.71	0.43	0.29	0.2	0.18	0.07
Corrected OD	2.22	1.22	0.64	0.36	0.22	0.13	0.11	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human GAP-43 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	93-105	102
Cell Lysates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human GAP-43 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108